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Product Name
Cytokeratin 8 antibody
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Description
Cytokeratin 8 Mouse Monoclonal antibody. Positive IHC detected in human stomach tissue, human stomach cancer tissue. Positive WB detected in A549 cells. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CARD2 antibody; CK 8 antibody; CK8 antibody; CYK8 antibody; Cytokeratin 8 antibody; K2C8 antibody; keratin 8 antibody; KRT8 antibody; Type II keratin Kb8 antibody
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Isotype
Mouse IgG3
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002273). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:107234(KRT8 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:107234(KRT8 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:107234(KRT8 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:107234(KRT8 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using Catalog No:107234(KRT8 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT8 is often paired with keratin 18 in vivo.
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