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Product Name
Cytokeratin 7-specific antibody
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Description
Cytokeratin 7-specific Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human breast cancer tissue, human heart tissue, human liver tissue, human lung cancer tissue, human spleen tissue, human stomach cancer tissue, human testis tissue, mouse lung tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse liver tissue, HepG2 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CK 7 antibody; CK7 antibody; Cytokeratin 7 antibody; K2C7 antibody; keratin 7 antibody; KRT7 antibody; Sarcolectin antibody; SCL antibody; Type II keratin Kb7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005556). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109812(KRT7-specific antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109812(KRT7-specific antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109812(KRT7-specific antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:109812(CK7-specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-CK7-specific (IP:Catalog No:109812, 4ug; Detection:Catalog No:109812 1:500) with mouse liver tissue lysate 4000ug.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.
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