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Product Name
Cytokeratin 4 antibody
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Description
Cytokeratin 4 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, human bladder tissue. Positive IHC detected in human cervix tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 57 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CK 4 antibody; CK4 antibody; CYK4 antibody; Cytokeratin 4 antibody; FLJ31692 antibody; keratin 4 antibody; KRT4 antibody; Type II keratin Kb4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 4 recombinant protein (Accession Number: BC042174). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Immunohistochemical of paraffin-embedded human cervix using Catalog No:109806(KRT4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervix using Catalog No:109806(KRT4 antibody) at dilution of 1:100 (under 40x lens)
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109806(KRT4 Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109806(KRT4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.
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