Cytokeratin 17 antibody

Cat.#: 109796

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Product Information

  • Product Name
    Cytokeratin 17 antibody
  • Documents
  • Description
    Cytokeratin 17 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue, human pancreas cancer tissue, human skin tissue. Positive WB detected in HeLa cells, A431 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 48 kDa
  • Tested applications
    ELISA, WB, FC, IP, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    39.1 antibody; CK 17 antibody; Cytokeratin 17 antibody; K17 antibody; keratin 17 antibody; KRT17 antibody; PC2 antibody; PCHC1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cytokeratin 17 recombinant protein (Accession Number: NM_000422). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109796(KRT17 antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109796(KRT17 antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 40x lens).

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109796(KRT17 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109796(KRT17 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    IP Result of anti-KRT17 (IP:Catalog No:109796, 4ug; Detection:Catalog No:109796 1:600) with HeLa cells lysate 1600ug.

    IP Result of anti-KRT17 (IP:Catalog No:109796, 4ug; Detection:Catalog No:109796 1:600) with HeLa cells lysate 1600ug.

    1X10^6 HeLa cells were stained with 0.2ug KRT17 antibody (Catalog No:109796, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug KRT17 antibody (Catalog No:109796, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"