-
Product Name
Cytokeratin 17 antibody
- Documents
-
Description
Cytokeratin 17 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human lung cancer tissue, human pancreas cancer tissue, human skin tissue. Positive WB detected in HeLa cells, A431 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 48 kDa
-
Tested applications
ELISA, WB, FC, IP, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
39.1 antibody; CK 17 antibody; Cytokeratin 17 antibody; K17 antibody; keratin 17 antibody; KRT17 antibody; PC2 antibody; PCHC1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Cytokeratin 17 recombinant protein (Accession Number: NM_000422). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109796(KRT17 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109796(KRT17 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109796(KRT17 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-KRT17 (IP:Catalog No:109796, 4ug; Detection:Catalog No:109796 1:600) with HeLa cells lysate 1600ug.
1X10^6 HeLa cells were stained with 0.2ug KRT17 antibody (Catalog No:109796, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
-
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"