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Product Name
Cytokeratin 16 antibody
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Description
Cytokeratin 16 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A375 cells, MCF7 cells. Positive IHC detected in human cervical cancer tissue, human cervix tissue, human skin tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CK 16 antibody; CK16 antibody; Cytokeratin 16 antibody; K16 antibody; K1CP antibody; keratin 16 antibody; KRT16 antibody; KRT16A antibody; NEPPK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 16 recombinant protein (Accession Number: NM_005557). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109795(KRT16 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109795(KRT16 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109795(KRT16 Antibody) at dilution of 1:200 (under 40x lens)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.
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