Mouse Cystatin E / CST6 (His Tag) recombinant protein

Mouse Cystatin E / CST6 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.

Cystatin E/M

ICHQ; 1110017E11RIK; CYSTATIN M; 7E11RIK; CYSTATIN E; CYSTATIN M/E; CST6; CYSTATIN 6; CYSTEINE PROTEINASE INHIBITOR; N28197

Q9D1B1

Cst6; mCG_11738

Human Cells

A DNA sequence encoding the extracellular domain of mouse CST6 (NP_082899.1) (Met 1-Ala 152) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse CST6 comprises 132 amino acids and has a calculated molecular mass of 15.2 kDa. As a result of glycosylation, the apparent molecular mass of rmCST6 is approximately 20 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate ZFR-AMC,R&D Systems, Catalog # ES009.
The IC50 is < 20 nM.