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Product Name
CYP46A1 antibody
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Description
CYP46A1 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human gliomas tissue, mouse brain tissue. Observed molecular weight by Western-blot: 57 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CH24H antibody; Cholesterol 24 hydroxylase antibody; CP46 antibody; CYP46 antibody; CYP46A1 antibody; CytOChrome P450 46A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP46A1 recombinant protein (Accession Number: NM_006668). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109702(CYP46A1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109702(CYP46A1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-CYP46A1 (IP:Catalog No:109702, 4ug; Detection:Catalog No:109702 1:500) with mouse brain tissue lysate 3200ug.
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Background
CYP46A1, also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)
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References
- Zhou M, Li P, Tan L, Qu S, Ying QL, Song H. Differentiation of mouse embryonic stem cells into hepatocytes induced by a combination of cytokines and sodium butyrate. Journal of cellular biochemistry. 109(3):606-14. 2010.
- Ali-Rahmani F, Grigson PS, Lee S, Neely E, Connor JR, Schengrund CL. H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment. Neurobiology of aging. 35(6):1511.e1-12. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"