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Product Name
CYP27A1 antibody
- Documents
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Description
CYP27A1 Rabbit Polyclonal antibody. Positive WB detected in L02 cells, HepG2 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CP27 antibody; CTX antibody; CYP27 antibody; CYP27A1 antibody; CytOChrome P 450C27/25 antibody; CytOChrome P450 27 antibody; Sterol 27 hydroxylase antibody; Vitamin D(3) 25 hydroxylase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP27A1 recombinant protein (Accession Number: NM_000784). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:109689(CYP27A1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109689(CYP27A1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109689(CYP27A1 antibody) at dilution of 1:100 (under 40x lens)
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Background
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"