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Product Name
CYP26B1 antibody
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Description
CYP26B1 Rabbit Polyclonal antibody. Positive IHC detected in human hepat℃irrhosis tissue, human heart tissue. Positive IF detected in HepG2 cells. Positive IP detected in L02 cells. Positive WB detected in human brain tissue, L02 cells. Observed molecular weight by Western-blot: 58-60 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CYP26A2 antibody; CYP26B1 antibody; CytOChrome P450 26A2 antibody; CytOChrome P450 26B1 antibody; CytOChrome P450RAI 2 antibody; DKFZp686G0638 antibody; P450RAI 2 antibody; P450RAI2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP26B1 recombinant protein (Accession Number: NM_019885). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109688(CYP26B1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:109688(CYP26B1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hepatocirrhosis using Catalog No:109688(CYP26B1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using CYP26B1 antibody Catalog No:109688 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CYP26B1 (IP:Catalog No:109688, 4ug; Detection:Catalog No:109688 1:500) with L02 cells lysate 2400ug.
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Background
CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).
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References
- Bowles J, Secker G, Nguyen C. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo. Developmental biology. 386(1):25-33. 2014.
- Login H, Butowt R, Bohm S. Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1. Brain structure & function. 220(4):2143-57. 2015.
- Login H, Håglin S, Berghard A, Bohm S. The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map. The Journal of neuroscience : the official journal of the Society for Neuroscience. 35(40):13807-18. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"