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Product Name
CYP19A1 antibody
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Description
CYP19A1 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue, human kidney tissue, human lung tissue, human ovary tissue, human skeletal muscle tissue, human spleen tissue, human testis tissue. Positive WB detected in A2780 cells, human testis tissue, mouse ovary tissue. Observed molecular weight by Western-blot: 49-55 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARO antibody; ARO1 antibody; Aromatase antibody; CPV1 antibody; CYAR antibody; CYP19 antibody; CYP19A1 antibody; CYPXIX antibody; CytOChrome P 450AROM antibody; CytOChrome P450 19A1 antibody; Estrogen synthase antibody; P 450AROM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CYP19A1 recombinant protein (Accession Number: BC022896). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:109682(CYP19A1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109682(CYP19A1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109682(CYP19A1 antibody) at dilution of 1:50 (under 40x lens)
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Background
CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).
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References
- Li J, Peng X, Zeng X. Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application. Scientific reports. 5:15286. 2015.
- O'Brien EE, Smeester BA, Michlitsch KS, Lee JH, Beitz AJ. Colocalization of aromatase in spinal cord astrocytes: differences in expression and relationship to mechanical and thermal hyperalgesia in murine models of a painful and a non-painful bone tumor. Neuroscience. 301:235-45. 2015.
- Wang H, Li G, Zhang J. Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure. Scientific reports. 5:13983. 2015.
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