Product Name

Cyclophilin B antibody

Documents

Description

Cyclophilin B Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue, human gliomas tissue, human pancreas cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, human skin tissue, mouse liver tissue, NIH/3T3 cells, Raji cells, U-937 cells. Observed molecular weight by Western-blot: 24 kDa

Tested applications

ELISA, IHC, WB, IF

Species reactivity

Human, Mouse; other species not tested.

Alternative names

Cyclophilin B antibody; CYP S1 antibody; CYPB antibody; PPIase B antibody; PPIB antibody; Rotamase B antibody; S cyclophilin antibody; SCYLP antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Cyclophilin B recombinant protein (Accession Number: NM_000942). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109674(PPIB antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109674(PPIB antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HeLa cells using Catalog No:109674(PPIB Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes.

References

Yue F, Wang LS, Xia L. Modulated T-complex protein 1 ζ and peptidyl-prolyl cis-trans isomerase B are two novel indicators for evaluating lymph node metastasis in colorectal cancer: Evidence from proteomics and bioinformatics. Proteomics. Clinical applications. 3(10):1225-35. 2009.
Watanabe A, Yoneda M, Ikeda F, Terao-Muto Y, Sato H, Kai C. CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells. Journal of virology. 84(9):4183-93. 2010.
van Dijk FS, Nesbitt IM, Zwikstra EH. PPIB mutations cause severe osteogenesis imperfecta. American journal of human genetics. 85(4):521-7. 2009.
Pyott SM, Schwarze U, Christiansen HE. Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes. Human molecular genetics. 20(8):1595-609. 2011.
Zhao JY, Liu CQ, Zhao HN. Synchronous detection of miRNAs, their targets and downstream proteins in transferred FFPE sections: applications in clinical and basic research. Methods (San Diego, Calif.). 58(2):156-63. 2012.
He H, Mou Z, Li W. Proteomic methods reveal cyclophilin A function as a host restriction factor against rotavirus infection. Proteomics. 13(7):1121-32. 2013.

Cyclophilin B antibody

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