Human Cyclin A1/CCNA1 (His Tag) recombinant protein

Human Cyclin A1/CCNA1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.

Cyclin-A1

MGC159139; CYCLIN-A1; CCNA1; CT146

P78396

CCNA1

Baculovirus-Insect Cells

A DNA sequence encoding the full length of human CCNA1 isoform a (NP_003905.1) (Met 1-Gln 465) was expressed, with a polyhistidine tag at the N-terminus.

Human

The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.

> 96 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 (Cat:501566) at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1 (Cat:500265). The EC₅₀ of biotinylated human CDK1 (Cat:500265) is 0.02-0.04 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 (Cat:501566) at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His (Cat:502274). The EC₅₀ of biotinylated human CDK2-His (Cat:502274）is 0.07-0.15 μg/ml.