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Anti-Ccna1 antibody

Cat.#: 176616

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Ccna1 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Ccna1
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human cyclin a1 aa 80-300 / 465.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:2,000

    FC: 1:100-1:500

  • Validations

    Fig1:; Western blot analysis of Cyclin A1 on rat testis tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of Cyclin A1 on rat testis tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody ( 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Fig2:; Flow cytometric analysis of Cyclin A1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Fig2:; Flow cytometric analysis of Cyclin A1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Background
    Q6AY13
  • References
    • Yang R. et. al. Functions of cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins. Mol. Cell. Biol. 19:2400-2407(1999).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"