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Product Name
CXCR5 Polyclonal Antibody
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Description
Polyclonal antibody to CXCR5
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Tested applications
WB, IF
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Species reactivity
Human
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Alternative names
CXCR5 antibody; BLR1 antibody; CD185 antibody; MDR15 antibody; C-X-C chemokine receptor type 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human CXCR5 (NP_001707.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CXCR5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CXCR5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - CXCR5 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CXCR5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"