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Product Name
CUL4B antibody
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Description
CUL4B Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, human testis tissue, mouse thymus tissue, SH-SY5Y cells, Y79 cells. Positive IF detected in Hela cells, HepG2 cells. Observed molecular weight by Western-blot: 102kd
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUL4B recombinant protein (Accession Number: NM_003588). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109747(CUL4B antibody) at dilution of 1:800
IP Result of anti-CUL4B (IP:Catalog No:109747, 3ug; Detection:Catalog No:109747 1:1000) with mouse brain tissue lysate 10000ug.
Immunofluorescent analysis of Hela cells, using CUL4B antibody Catalog No:109747 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.
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