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Product Name
CUEDC2 antibody
- Documents
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Description
CUEDC2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human kidney tissue, human brain tissue. Positive IF detected in Hela cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bA18I14.5 antibody; C10orf66 antibody; CUE domain containing 2 antibody; CUEDC2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CUEDC2 recombinant protein (Accession Number: BC000262). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109738(CUEDC2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human brain using Catalog No:109738(CUEDC2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109738(CUEDC2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CUEDC2 antibody Catalog No:109738 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CUEDC2 (IP:Catalog No:109738, 4ug; Detection:Catalog No:109738 1:500) with mouse brain tissue lysate 4000ug.
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Background
CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.
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