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  • CTP synthase antibody
  • CTP synthase antibody
  • CTP synthase antibody
  • CTP synthase antibody
  • CTP synthase antibody
  • CTP synthase antibody

CTP synthase antibody

Cat.#: 109728

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Product Information

  • Product Name
    CTP synthase antibody
  • Documents
  • Description
    CTP synthase Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive FC detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 67 kDa
  • Tested applications
    ELISA, WB, IF, FC, IP, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CTP synthase antibody; CTP synthase 1 antibody; CTP synthetase 1 antibody; CTPS antibody; UTP ammonia ligase 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CTP synthase recombinant protein (Accession Number: BC009408). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:50-1:500

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109728(CTPS antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109728(CTPS antibody) at dilution of 1:500

    Immunofluorescent analysis of HepG2 cells, using CTPS antibody Catalog No:109728 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using CTPS antibody Catalog No:109728 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-CTPS (IP:Catalog No:109728, 4ug; Detection:Catalog No:109728 1:500) with HeLa cells lysate 1200ug.

    IP Result of anti-CTPS (IP:Catalog No:109728, 4ug; Detection:Catalog No:109728 1:500) with HeLa cells lysate 1200ug.

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 10x lens).

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 40x lens).

    Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 40x lens).

    1X10^6 HepG2 cells were stained with 0.2ug CTPS antibody (Catalog No:109728, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HepG2 cells were stained with 0.2ug CTPS antibody (Catalog No:109728, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis(PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)
  • References
    • Keppeke GD, Calise SJ, Chan EK, Andrade LE. Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction. Journal of genetics and genomics = Yi chuan xue bao. 42(6):287-99. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"