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Product Name
CTP synthase antibody
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Description
CTP synthase Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive FC detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 67 kDa
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Tested applications
ELISA, WB, IF, FC, IP, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CTP synthase antibody; CTP synthase 1 antibody; CTP synthetase 1 antibody; CTPS antibody; UTP ammonia ligase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CTP synthase recombinant protein (Accession Number: XM_024453553). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109728(CTPS antibody) at dilution of 1:500
Immunofluorescent analysis of HepG2 cells, using CTPS antibody Catalog No:109728 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CTPS (IP:Catalog No:109728, 4ug; Detection:Catalog No:109728 1:500) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 10x lens).
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:109728(CTPS Antibody) at dilution of 1:100 (under 40x lens).
1X10^6 HepG2 cells were stained with 0.2ug CTPS antibody (Catalog No:109728, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis(PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)
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References
- Keppeke GD, Calise SJ, Chan EK, Andrade LE. Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction. Journal of genetics and genomics = Yi chuan xue bao. 42(6):287-99. 2015.
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