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CSTF1 antibody

Cat.#: 109616

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Special Price 239.0 USD

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Product Information

  • Product Name
    CSTF1 antibody
  • Documents
  • Description
    CSTF1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, mouse brain tissue, mouse kidney tissue, NIH/3T3 cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 50-58 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CF 1 50 kDa subunit antibody; Cleavage stimulation factor antibody; CstF 50 antibody; CSTF 50 kDa subunit antibody; CSTF1 antibody; CstFp50 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CSTF1 recombinant protein (Accession Number: XM_011528600). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109616(CSTF1 antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109616(CSTF1 antibody) at dilution of 1:1000

    IP Result of anti-CSTF1 (IP:Catalog No:109616, 2ug; Detection:Catalog No:109616 1:500) with NIH/3T3 cells lysate 2500ug.

    IP Result of anti-CSTF1 (IP:Catalog No:109616, 2ug; Detection:Catalog No:109616 1:500) with NIH/3T3 cells lysate 2500ug.

  • Background
    Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.
  • References
    • Uguen P, Murphy S. The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing. The EMBO journal. 22(17):4544-54. 2003.
    • Nazeer FI, Devany E, Mohammed S. p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex. Oncogene. 30(27):3073-83. 2011.
    • Dai J, Zhan C, Xu W. Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis. Andrology. 3(5):967-78. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"