-
Product Name
CSPP1 antibody
- Documents
-
Description
CSPP1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 141kd
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
CSPP antibody; CSPP1 antibody; FLJ22490 antibody; FLJ38886 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CSPP1 recombinant protein (Accession Number: BC029445). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109606(CSPP1 antibody) at dilution of 1:200
Immunofluorescent analysis of Hela cells, using CSPP1 antibody Catalog No:109606 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
-
References
- Sternemalm J, Russnes HG, Zhao X. Nuclear CSPP1 expression defined subtypes of basal-like breast cancer. British journal of cancer. 111(2):326-38. 2014.
- Sternemalm J, Geimer S, Frikstad KA, Schink KO, Stokke T, Patzke S. CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation. PloS one. 10(8):e0134789. 2015.
- Patzke S, Redick S, Warsame A. CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation. Molecular biology of the cell. 21(15):2555-67. 2010.
- Tuz K, Bachmann-Gagescu R, O'Day DR. Mutations in CSPP1 cause primary cilia abnormalities and Joubert syndrome with or without Jeune asphyxiating thoracic dystrophy. American journal of human genetics. 94(1):62-72. 2014.
- Akizu N, Silhavy JL, Rosti RO. Mutations in CSPP1 lead to classical Joubert syndrome. American journal of human genetics. 94(1):80-6. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"