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Product Name
CSNK2A1 antibody
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Description
CSNK2A1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Casein kinase II subunit alpha antibody; CK II alpha antibody; CK2A1 antibody; CKII antibody; CSNK2A1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CSNK2A1 recombinant protein (Accession Number: NM_001362770). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109603(CSNK2A1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109603(CSNK2A1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109603(CSNK2A1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CSNK2A1 (IP:Catalog No:109603, 4ug; Detection:Catalog No:109603 1:1000) with HeLa cells lysate 2800ug.
Immunofluorescent analysis of HeLa cells using Catalog No:109603(CSNK2A1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. this enzyme is composed of an α subunit (37 to 44kDa) and aβsubunit (24 to 28 kDa) and exists as an α2β2 tetramer.(PMID:1448100). Casein kinase 2 has been found to be inccreased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
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