-
Product Name
CSNK1G1 antibody
- Documents
-
Description
CSNK1G1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, COS-7 cells. Positive IP detected in Jurkat cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 49 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
-
Alternative names
casein kinase 1 antibody; gamma 1 antibody; CKI gamma 1 antibody; CSNK1G1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of CSNK1G1 recombinant protein (Accession Number: BC017236). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109602(CSNK1G1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:109602(CSNK1G1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:109602(CSNK1G1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CSNK1G1 (IP:Catalog No:109602, 4ug; Detection:Catalog No:109602 1:300) with Jurkat cells lysate 2800ug.
-
Background
The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.
-
References
- Wang Y, Hu L, Tong X, Ye X. Casein kinase 1γ1 inhibits the RIG-I/TLR signaling pathway through phosphorylating p65 and promoting its degradation. Journal of immunology (Baltimore, Md. : 1950). 192(4):1855-61. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"