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Product Name
Cryptochrome 2 antibody
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Description
Cryptochrome 2 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in Y79 cells. Positive IF detected in SH-SY5Y cells. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CRY2 antibody; CryptOChrome 2 antibody; FLJ10332 antibody; HCRY2 antibody; KIAA0658 antibody; PHLL2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Crypt℃hrome 2 recombinant protein (Accession Number: NM_021117). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:50-1:500
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:109589(CRY2 antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using Catalog No:109589(CRY2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CRY2 represents cryptochrome 2 (photolyase-like)
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References
- Lee Y, Jang AR, Francey LJ, Sehgal A, Hogenesch JB. KPNB1 mediates PER/CRY nuclear translocation and circadian clock function. eLife. 4:. 2015.
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