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Product Name
CROP antibody
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Description
CROP Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HepG2 cells, mouse brain tissue. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue, human ovary tissue. Observed molecular weight by Western-blot: 55-58 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CRE assOCiated protein 1 antibody; CREAP 1 antibody; CREAP1 antibody; CROP antibody; Luc7 like protein 3 antibody; LUC7A antibody; LUC7L3 antibody; O48 antibody; OA48 18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CROP recombinant protein (Accession Number: BC056409). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109576(CROP antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109576(CROP antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-CROP (IP:Catalog No:109576, 4ug; Detection:Catalog No:109576 1:500) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:109576(CROP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cisplatin resistance-associated overexpressed protein (CROP), a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.
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