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Product Name
CRM1 antibody
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Description
CRM1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HT-1080 cells. Positive IP detected in HeLa cells. Positive IHC detected in human heart tissue, human kidney tissue, human pancreas tissue. Observed molecular weight by Western-blot: 100-110 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CRM1 antibody; DKFZp686B1823 antibody; Exp1 antibody; Exportin 1 antibody; XPO1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CRM1 recombinant protein (Accession Number: XM_047445760). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109568(XPO1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:109568(XPO1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:109568(XPO1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-XPO1 (IP:Catalog No:109568, 4ug; Detection:Catalog No:109568 1:500) with HeLa cells lysate 2800ug.
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Background
CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).
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