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Product Name
CRHR2 Polyclonal Antibody
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Description
Polyclonal antibody to CRHR2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CRHR2 antibody; CRF-RB antibody; CRF2 antibody; CRFR2 antibody; HM-CRF antibody; corticotropin-releasing factor receptor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human CRHR2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - CRHR2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CRHR2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CRHR2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using CRHR2 antibody (40x lens).
Immunohistochemistry - CRHR2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using CRHR2 antibody (40x lens).
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Background
G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"