CREST antibody

Cat.#: 109555

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Product Information

  • Product Name
    CREST antibody
  • Documents
  • Description
    CREST Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human brain tissue. Positive WB detected in K-562 cells, COLO 320 cells, human heart tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, IHC, IP, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    CREST antibody; KIAA0693 antibody; LP2261 antibody; SS18 like protein 1 antibody; SS18L1 antibody; SYT homolog 1 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of CREST recombinant protein (Accession Number: NM_015558). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109555(CREST Antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109555(CREST Antibody) at dilution of 1:1000

    IP Result of anti-CREST (IP:Catalog No:109555, 3ug; Detection:Catalog No:109555 1:1000) with mouse brain tissue lysate 6000ug.

    IP Result of anti-CREST (IP:Catalog No:109555, 3ug; Detection:Catalog No:109555 1:1000) with mouse brain tissue lysate 6000ug.

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109555(CREST antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109555(CREST antibody) at dilution of 1:50 (under 10x lens)

  • Background
    SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP
  • References
    • Chesi A, Staahl BT, Jovičić A. Exome sequencing to identify de novo mutations in sporadic ALS trios. Nature neuroscience. 16(7):851-5. 2013.
    • Staahl BT, Tang J, Wu W. Kinetic analysis of npBAF to nBAF switching reveals exchange of SS18 with CREST and integration with neural developmental pathways. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(25):10348-61. 2013.
    • Kukharsky MS, Quintiero A, Matsumoto T. Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis. Molecular neurodegeneration. 10:20. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"