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Product Name
CREST antibody
- Documents
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Description
CREST Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human brain tissue. Positive WB detected in K-562 cells, COLO 320 cells, human heart tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, IHC, IP, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CREST antibody; KIAA0693 antibody; LP2261 antibody; SS18 like protein 1 antibody; SS18L1 antibody; SYT homolog 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CREST recombinant protein (Accession Number: NM_015558). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:109555(CREST Antibody) at dilution of 1:1000
IP Result of anti-CREST (IP:Catalog No:109555, 3ug; Detection:Catalog No:109555 1:1000) with mouse brain tissue lysate 6000ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109555(CREST antibody) at dilution of 1:50 (under 10x lens)
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Background
SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP
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References
- Chesi A, Staahl BT, Jovičić A. Exome sequencing to identify de novo mutations in sporadic ALS trios. Nature neuroscience. 16(7):851-5. 2013.
- Staahl BT, Tang J, Wu W. Kinetic analysis of npBAF to nBAF switching reveals exchange of SS18 with CREST and integration with neural developmental pathways. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(25):10348-61. 2013.
- Kukharsky MS, Quintiero A, Matsumoto T. Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis. Molecular neurodegeneration. 10:20. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"