Creatine Kinase M-Specific antibody

Creatine Kinase M-Specific antibody

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• Material Safety Data Sheets (MSDS)

Creatine Kinase M-Specific Rabbit Polyclonal antibody. Positive IHC detected in human skeletal muscle tissue, rat heart tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse heart tissue, mouse brain tissue, mouse small intestine tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 43 kDa

ELISA, WB, IHC, IF, IP

Human,Mouse,Rat; other species not tested.

CKMM antibody; Creatine Kinase MM antibody; Creatine Kinase M-Specific antibody; Creatine kinase M chain antibody; Creatine kinase M type antibody; creatine kinase antibody; muscle antibody; M CK antibody

Rabbit IgG

This antibody was obtained by immunization of Peptide (Accession Number: NM_001824). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42 kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185).