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Product Name
CPN1 antibody
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Description
CPN1 Rabbit Polyclonal antibody. Positive WB detected in Raji cells, COLO 320 cells. Positive FC detected in HeLa cells. Positive IF detected in COLO 320 cells. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACBP antibody; Anaphylatoxin inactivator antibody; Arginine carboxypeptidase antibody; CPN antibody; CPN1 antibody; FLJ40792 antibody; Kininase 1 antibody; Lysine carboxypeptidase antibody; Plasma carboxypeptidase B antibody; SCPN antibody; Serum carboxypeptidase N antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CPN1 recombinant protein (Accession Number: NM_001308). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Raji cells were subjected to SDS PAGE followed by western blot with Catalog No:109514(CPN1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109514(CPN1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109514(CPN1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of COLO 320 cells using Catalog No:109514(CPN1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HeLa cells were stained with 0.2ug CPN1 antibody (Catalog No:109514, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CPN1 represents carboxypeptidase N, polypeptide 1
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