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Product Name
CPLX3 antibody
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Description
CPLX3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse brain tissue, human brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 20-23 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
complexin 3 antibody; Complexin III antibody; CPLX3 antibody; CPX III antibody; CPXIII antibody; FLJ13993 antibody; Nbla11589 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CPLX3 recombinant protein (Accession Number: NM_001030005). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109512(CPLX3 antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using CPLX3 antibody Catalog No:109512 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-CPLX3 (IP:Catalog No:109512, 3ug; Detection:Catalog No:109512 1:300) with mouse brain tissue lysate 6000ug.
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Background
CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.
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References
- Wang Z, Wei X, Liu K. NOX2 deficiency ameliorates cerebral injury through reduction of complexin II-mediated glutamate excitotoxicity in experimental stroke. Free radical biology & medicine. 65:942-51. 2013.
- Pedraza M, Hoerder-Suabedissen A, Albert-Maestro MA, Molnár Z, De Carlos JA. Extracortical origin of some murine subplate cell populations. Proceedings of the National Academy of Sciences of the United States of America. 111(23):8613-8. 2014.
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