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Product Name
Anti-CPLX3 antibody
- Documents
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Description
Rabbit Polyclonal to Human CPLX3
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Tested applications
ELISA, IHC-P
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Species reactivity
Human CPLX3 / Complexin 3
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Alternative names
complexin 3 antibody; CPLX3 antibody; Nbla11589 antibody; CPX-III antibody; CPXIII antibody; complexin III antibody; complexin 3 antibody; Cplx3 antibody; BC016632 antibody; CpxIII antibody; ERGIC-53L antibody; ERGL antibody; Lamn1l antibody; Lman1l antibody; CPX III antibody; complexin III antibody; lectin antibody; mannose-binding antibody; 1 like antibody; ERGL antibody; CpxIII antibody; Lamn1l antibody; Lman1l antibody; BC016632 antibody; ERGIC-53L antibody; CPXIII antibody; CPX-III antibody; Nbla11589 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CPLX3 / Complexin 3 . CPLX3 / Complexin 3 specific IgG was purified by Human CPLX3 / Complexin 3 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human CPLX3 / Complexin 3.
IHC-P: 0.1-2 μg/mL
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Validations
CPLX3 / Complexin 3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
CPLX3, also known as complexin 3, belongs to the complexin/synaphin family. As a SNARE-binding protein, complexin (CPX), can act either as a facilitator or as an inhibitor of membrane fusion, constituting a controversial dilemma. CPX acts sequentially on assembling SNAREpins, first facilitating zippering by nearly doubling the distance at which v- and t-SNAREs can engage and then clamping them into a half-zippered fusion-incompetent state. Specifically, the central helix of CPX allows SNAREs to form this intermediate energetic state at 9-15 nm but not when the bilayers are closer than 9 nm. Stabilizing the activated-clamped state at separations of less than 9 nm requires the accessory helix of CPX, which prevents membrane-proximal assembly of SNAREpins. CPLX3 binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"