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Product Name
COX5A antibody
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Description
COX5A Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human liver tissue, L02 cells, mouse brain tissue. Positive IF detected in HeLa cells. Positive IHC detected in human gliomas tissue, human colon tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 13 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COX antibody; COX VA antibody; COX5A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COX5A recombinant protein (Accession Number: NM_004255). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109491(COX5A Antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:109491(COX5A antibody) at dilution of 1:50 (under 10x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109491(COX5A Antibody) at dilution of 1:800
Immunofluorescent analysis of HeLa cells using Catalog No:109491(COX5A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
COX5A(Cytochrome c oxidase subunit 5A,mitochondrial)is also named as COXVA and belongs to the cytochrome c oxidase subunit 5A family.This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).
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References
- Uddin M, Opazo JC, Wildman DE. Molecular evolution of the cytochrome c oxidase subunit 5A gene in primates. BMC evolutionary biology. 8:8. 2008.
- Osunkoya AO, Yin-Goen Q, Phan JH. Diagnostic biomarkers for renal cell carcinoma: selection using novel bioinformatics systems for microarray data analysis. Human pathology. 40(12):1671-8. 2009.
- Oliva CR, Nozell SE, Diers A. Acquisition of temozolomide chemoresistance in gliomas leads to remodeling of mitochondrial electron transport chain. The Journal of biological chemistry. 285(51):39759-67. 2010.
- Gong YY, Liu YY, Li J. Hypermethylation of Cox5a promoter is associated with mitochondrial dysfunction in skeletal muscle of high fat diet-induced insulin resistant rats. PloS one. 9(12):e113784. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"