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Product Name
COMT Polyclonal Antibody
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Description
Polyclonal antibody to COMT
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Tested applications
WB, IP
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Species reactivity
Human, Mouse
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Alternative names
COMT antibody; HEL-S-98n antibody; catechol O-methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-221 of human COMT (NP_009294.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IP 1:50 - 1:100 -
Validations
Western blot - COMT Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using COMT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunoprecipitation - COMT Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug COMT antibody . Western blot was performed from the immunoprecipitate using COMT antibody at a dilition of 1:1000.
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Background
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"