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Product Name
COMMD1 antibody
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Description
COMMD1 Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human liver cancer tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 21kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C2orf5 antibody; COMMD1 antibody; MURR1 antibody; Protein Murr1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COMMD1 recombinant protein (Accession Number: NM_152516). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109443(COMMD1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109443(COMMD1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109443(COMMD1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-COMMD1 (IP:Catalog No:109443, 3ug; Detection:Catalog No:109443 1:500) with mouse brain tissue lysate 5200ug.
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Background
COMMD1(COMM domain-containing protein 1), also named MURR1, is implicated in copper homeostasis by binding one copper ion per monomer. COMMD1 was reported to accelerate the ubiquitination and degradation of NF-kappa-B subunits, its deficiency exerts enhanced NF-kappa-B mediated celluar response. Ubiquitinated by XIAP (X-linked inhibitor of apoptosis), COMMD1 undergoes proteasomal degradation. SOD1 activity can be downregulated by COMMD1.
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References
- Miyayama T, Hiraoka D, Kawaji F, Nakamura E, Suzuki N, Ogra Y. Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line. The Biochemical journal. 429(1):53-61. 2010.
- Yeh DW, Chen YS, Lai CY. Downregulation of COMMD1 by miR-205 promotes a positive feedback loop for amplifying inflammatory- and stemness-associated properties of cancer cells. Cell death and differentiation. 2015.
- Drévillon L, Tanguy G, Hinzpeter A. COMMD1-mediated ubiquitination regulates CFTR trafficking. PloS one. 6(3):e18334. 2011.
- Han M, Lin Z, Zhang Y. The alteration of copper homeostasis in inflammation induced by lipopolysaccharides. Biological trace element research. 154(2):268-74. 2013.
- de Becdelièvre A, Rocca J, Aissat A. COMMD1 modulates noxious inflammation in cystic fibrosis. The international journal of biochemistry & cell biology. 45(11):2402-9. 2013.
- Bartuzi P, Wijshake T, Dekker DC. A cell-type-specific role for murine Commd1 in liver inflammation. Biochimica et biophysica acta. 1842(11):2257-65. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"