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Product Name
CIT antibody
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Description
CIT Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive WB detected in A431 cells, HeLa cells, mouse brain tissue. Observed molecular weight by Western-blot: 230kd,177kd
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Tested applications
ELISA, WB, IF
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
CIT antibody; STK21 antibody; KIAA0949 antibody; Citron Rho interacting kinase antibody; CRIK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_007174). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:109323(CIT antibody) at dilution of 1:300
Immunofluorescent analysis of HepG2 cells, using CIT antibody Catalog No:109323 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoformes of CIT.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"