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Product Name
CIRBP antibody
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Description
CIRBP Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A549 cells, Cold treated HepG2 cells, HEK-293 cells, human brain tissue, mouse brain tissue, mouse testis tissue, PC-13 cells, Y79 cells. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human breast cancer tissue, human placenta tissue. Observed molecular weight by Western-blot: 19 kDa
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Tested applications
ELISA, IHC, IP, IF, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
A18 hnRNP antibody; A18HNRNP antibody; CIRBP antibody; CIRP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CIRBP recombinant protein (Accession Number: NM_001280). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of CIRBP antibody (Catalog No:109319, 1:500) with si-control and si-CIRBP transfected A549 cell.
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109319(CIRBP antibody) at dilution of 1:1000
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109319(CIRBP Antibody) at dilution of 1:600
IP Result of anti-CIRBP (IP:Catalog No:109319, 3ug; Detection:Catalog No:109319 1:500) with HepG2 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109319(CIRBP antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109319(CIRBP Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.
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References
- Xia ZP, Zheng XM, Zheng H, Liu XJ, Liu GY, Wang XH. Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes. Asian journal of andrology. 14(6):884-9. 2012.
- Brochu C, Cabrita MA, Melanson BD. NF-κB-dependent role for cold-inducible RNA binding protein in regulating interleukin 1β. PloS one. 8(2):e57426. 2013.
- Guo X, Wu Y, Hartley RS. Cold-inducible RNA-binding protein contributes to human antigen R and cyclin E1 deregulation in breast cancer. Molecular carcinogenesis. 49(2):130-40. 2010.
- Saito K, Fukuda N, Matsumoto T. Moderate low temperature preserves the stemness of neural stem cells and suppresses apoptosis of the cells via activation of the cold-inducible RNA binding protein. Brain research. 1358:20-9. 2010.
- Lee HN, Ahn SM, Jang HH. Cold-inducible RNA-binding protein, CIRP, inhibits DNA damage-induced apoptosis by regulating p53. Biochemical and biophysical research communications. 464(3):916-21. 2015.
- Rzechorzek NM, Connick P, Patani R, Selvaraj BT, Chandran S. Hypothermic Preconditioning of Human Cortical Neurons Requires Proteostatic Priming. EBioMedicine. 2(6):528-35. 2015.
- Yu Y, Xu X, Liu L. Progranulin deficiency leads to severe inflammation, lung injury and cell death in a mouse model of endotoxic shock. Journal of cellular and molecular medicine. 20(3):506-17. 2016.
- Artero-Castro A, Callejas FB, Castellvi J. Cold-inducible RNA-binding protein bypasses replicative senescence in primary cells through extracellular signal-regulated kinase 1 and 2 activation. Molecular and cellular biology. 29(7):1855-68. 2009.
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