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Product Name
Cingulin antibody
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Description
Cingulin Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive WB detected in mouse kidney tissue, mouse small intestine tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 136-150 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGN antibody; Cingulin antibody; DKFZp779N1112 antibody; FLJ39281 antibody; KIAA1319 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cingulin recombinant protein (Accession Number: BC146657). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109317(CGN antibody) at dilution of 1:500
IP Result of anti-CGN (IP:Catalog No:109317, 4ug; Detection:Catalog No:109317 1:800) with mouse kidney tissue lysate 4800ug.
Immunofluorescent analysis of A431 cells using Catalog No:109317(CGN Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.
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References
- Ducommun S, Deak M, Sumpton D. Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: identification of mitochondrial fission factor as a new AMPK substrate. Cellular signalling. 27(5):978-88. 2015.
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