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Product Name
Chromogranin A antibody
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Description
Chromogranin A Mouse Monoclonal antibody. Positive WB detected in rat pancreas tissue, human adrenal gland tissue, Transfected HEK-293 cells. Positive IHC detected in human pancreas tissue, human colon tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
CGA antibody; CHGA antibody; Chromogranin A antibody; Pituitary secretory protein I antibody; SP I antibody; Vasostatin I Vasostatin 2 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Chromogranin A recombinant protein (Accession Number: NM_001275). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
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Validations
rat pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:107157(CHGA antibody) at dilution of 1:1000
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107157(CHGA Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107157(CHGA Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107157(CHGA Antibody) at dilution of 1:200 (under 40x lens).
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Background
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
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