Human CHI3L1/YKL40 (His Tag) recombinant protein

Human CHI3L1/YKL40 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Chitinase-3-like protein 1 (CHI3L1) is a secreted heparin-binding glycoprotein whose expression is associated with vascular smooth muscle cell migration. CHI3L1 is expressed at high levels in postconfluent nodular VSMC cultures and at low levels in subconfluent proliferating cultures. CHI3L1 is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyto / macrophage markers, its expression is absent in monocytes and strong induced during late stages of human macrophage differentiation. Elevated levels of CHI3L1 are associated with disorders exhibiting increased connective tissue turnover, such as rheum atoid, arthritis, osteoarthritis, scleroderma, and cirrhosis of liver, but is produced in cartilage from old donors or patiens with osteoarthritis. CHI3L1 is abnormally expressed in the hippocampus of subjects with schizophrenia and may be involved in the cellular response to various environmental events that are reported to increase the risk of schizophrenia.

Chitinase 3-like 1 (Cartilage glycoprotein-39), isoform CRA_a

FLJ38139; DKFZP686N19119; GP39; ASRT7; BRP39; HC-GP39; AW208766; HCGP-3P; CHI3L1; YKL40; YYL-40

P36222

CHI3L1; hCG_24326

Human Cells

A DNA sequence encoding the human CHI3L1 (NP_001267.2) (Met 1-Thr 383) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human CHI3L1 consists of 373 amino acids and has a predicted molecular mass of 42 kDa as estimated in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of Fadu human squamous cell carcinoma cells (ATCC:HTB43). When cells are added to recombinant human CHI3L1 coated plates (1.5 μg/ml, 100 μl/well), approximately 65%-75% will adhere specifically after 30 minutes at 37℃.