Anti-CHI3L1 antibody

Cat.#: 175094

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Product Information

  • Product Name
    Anti-CHI3L1 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to CHI3L1
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human
  • Alternative names
    GP39 antibody; ASRT7 antibody; GP-39 antibody; YK-40 antibody; YKL40 antibody; CGP-39 antibody; YKL-40 antibody; YYL-40 antibody; HC-gp39 antibody; HCGP-3P antibody; hCGP-39 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antigen of this antibody was recombinant protein within extracellular domain of human chi3l1.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB:1:500-1:2,000

    IHC-P:1:50-1:200

  • Validations

    Fig1:; Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western blot analysis of YKL-40/CHI3L1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2:; Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40/CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Fig2:; Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40/CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH; 2; O and PBS, and then probed with the primary antibody ( 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Background
  • References
    • Qiu QC. et. al. CHI3L1 promotes tumor progression by activating TGF-β signaling pathway in hepatocellular carcinoma. Sci Rep. 2018 Oct
    • Abd El-Fattah AA. et. al. Single Nucleotide Polymorphism in SMAD7 and CHI3L1 and Colorectal Cancer Risk. Mediators Inflamm. 2018 Oct

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"