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Product Name
CHD1 Polyclonal Antibody
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Description
Polyclonal antibody to CHD1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHD1 antibody; chromodomain-helicase-DNA-binding protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200 -
Validations
Western blot - CHD1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHD1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - CHD1 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and CHD1 knockout 293T cells, using CHD1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CHD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CHD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using CHD1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CHD1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CHD1 antibody at dilution of 1:100 (40x lens).
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Background
ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3. Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"