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Product Name
CHCHD2 antibody
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Description
CHCHD2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HT-1080 cells, human adrenal gland tissue. Observed molecular weight by Western-blot: 16-18 kDa
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Tested applications
ELISA, IF, IP, WB
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Species reactivity
Human; other species not tested.
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Alternative names
C7orf17 antibody; CHCHD2 antibody; NS2TP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CHCHD2 recombinant protein (Accession Number: NM_016139). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody Catalog No:109212 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:109212(CHCHD2 Antibody) at dilution of 1:600
IP Result of anti-CHCHD2 (IP:Catalog No:109212, 4ug; Detection:Catalog No:109212 1:500) with HepG2 cells lysate 2800ug.
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Background
CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. The function of the CHCH domain is not well understood, and the few characterized proteins that harbor this domain have diverse functions. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD).
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References
- Aras S, Bai M, Lee I, Springett R, Hüttemann M, Grossman LI. MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism. Mitochondrion. 20:43-51. 2015.
- Funayama M, Ohe K, Amo T. CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study. The Lancet. Neurology. 14(3):274-82. 2015.
- Li HY, Zhang LK, Zhu XJ. Analysis of EV71 infection progression using triple-SILAC-based proteomics approach. Proteomics. 15(21):3629-43. 2015.
- Feyeux M, Bourgois-Rocha F, Redfern A. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Human molecular genetics. 21(17):3883-95. 2012.
- Aras S, Pak O, Sommer N. Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2. Nucleic acids research. 41(4):2255-66. 2013.
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