CHAF1A antibody

CHAF1A antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

CHAF1A Rabbit Polyclonal antibody. Positive IHC detected in human cervix tissue. Positive WB detected in Jurkat cells, HeLa cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 150 kDa

ELISA, IHC, WB, IP

Human, Mouse; other species not tested.

CAF antibody; CAF 1 antibody; CAF 1 subunit A antibody; CAF I 150 kDa subunit antibody; CAF I p150 antibody; CAF1 antibody; CAF1B antibody; CAF1P150 antibody; CHAF1A antibody; hp150 antibody; P150 antibody

Rabbit IgG

This antibody was obtained by immunization of CHAF1A recombinant protein (Accession Number: NM_005483). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248].

• Begum NA, Stanlie A, Nakata M, Akiyama H, Honjo T. The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation. The Journal of biological chemistry. 287(39):32415-29. 2012.