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Product Name
Anti-p150 CAF1/CAF Rabbit antibody
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Description
p150 CAF1/CAF Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human
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Alternative names
CAF1; P150; CAF-1; CAF1B; CAF1P150 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human p150 CAF1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
ICC/IF: 1/20
FC: 1/200-1/1000
IP: 1/20
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Validations
Immunocytochemistry of p150 CAF1(green) in Hela cells using p150 CAF1 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of p150 CAF1 in K562 cell lysates using p150 CAF1 Rabbit pAb(1:1000 diluted).Predicted band size:107kDa.Observed band size:150kDa.
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Background
Swiss-Prot Acc.Q13111.Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"