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Product Name
CEP97 antibody
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Description
CEP97 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells. Positive IF detected in MDCK cells. Positive IHC detected in human breast cancer tissue, human liver tissue. Observed molecular weight by Western-blot: 130-140 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
2810403B08Rik antibody; centrosomal protein 97kDa antibody; Centrosomal protein of 97 kDa antibody; CEP97 antibody; FLJ23047 antibody; FLJ26462 antibody; LRRIQ2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CEP97 recombinant protein (Accession Number: NM_024548). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109188(CEP97 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:109188(CEP97 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-CEP97 (IP:Catalog No:109188, 4ug; Detection:Catalog No:109188 1:1000) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of MDCK cells using Catalog No:109188(CEP97 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.
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References
- Prosser SL, Morrison CG. Centrin2 regulates CP110 removal in primary cilium formation. The Journal of cell biology. 208(6):693-701. 2015.
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