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Product Name
CEP290 antibody
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Description
CEP290 Rabbit Polyclonal antibody. Positive IHC detected in human placenta tissue. Positive IF detected in HeLa cells. Positive WB detected in HEK-293 cells, HeLa cells, MCF7 cells, PC-3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 290 kDa, 150-180 kDa, 80 kDa,60 kDa
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Tested applications
ELISA, IHC, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
3H11Ag antibody; BBS14 antibody; Cancer/testis antigen 87 antibody; centrosomal protein 290kDa antibody; Centrosomal protein of 290 kDa antibody; CEP290 antibody; CT87 antibody; FLJ13615 antibody; FLJ21979 antibody; JBTS5 antibody; JBTS6 antibody; KIAA0373 antibody; LCA10 antibody; MKS4 antibody; NephrOCystin 6 antibody; rd16 antibody; SLSN6 antibody; Tumor antigen se2 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CEP290 recombinant protein (Accession Number: BC008641). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109179(CEP290 antibody) at dilution of 1:500
IP result of anti-CEP290(Catalog No:109179 for IP and Detection).
Immunohistochemical of paraffin-embedded human placenta using Catalog No:109179(CEP290 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:109179(CEP290 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290’s function is critical for IFT—in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining.
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