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Product Name
Centromere protein R antibody
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Description
Centromere protein R Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IHC detected in human colon cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
Beta 3 endonexin antibody; CENP R antibody; CENPR antibody; Centromere protein R antibody; HSU37139 antibody; ITGB3BP antibody; NRIF3 antibody; TAP20 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Centromere protein R recombinant protein (Accession Number: NM_014288). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109262(ITGB3BP antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109262(ITGB3BP antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:109262(Centromere protein R Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ITGB3BP, also named as CENPR and NRIF3; is a transcription coregulator that can have both coactivator and corepressor functions. ITGB3BP acts as a coactivator for estrogen receptor alpha. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. ITGB3BP induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP has five isoforms with MW 7 kDa, 13 kDa and 19-25 kDa.
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References
- Talukder AH, Li DQ, Manavathi B, Kumar R. Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation. Oncogene. 27(39):5233-42. 2008.
- Tinnikov AA, Yeung KT, Das S, Samuels HH. Identification of a novel pathway that selectively modulates apoptosis of breast cancer cells. Cancer research. 69(4):1375-82. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"