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Product Name
Centrin 2 antibody
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Description
Centrin 2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, rat brain tissue. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CALT antibody; Caltractin isoform 1 antibody; CEN2 antibody; Centrin 2 antibody; centrin 2 antibody; centrin2 antibody; centrin antibody; EF hand protein antibody; CETN2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Centrin 2 recombinant protein (Accession Number: NM_004344). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109260(Centrin 2 Antibody) at dilution of 1:600
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109260(Centrin 2 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human brain using Catalog No:109260(CETN2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:109260(CETN2 antibody) at dilution of 1:50 (under 40x lens)
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Background
Centrin 2 is also named as CALT, or CEN2. It plays a fundamental role in microtubule-organizing center structure and function. Oxidizing radiation induces polymerization of centrin 2 into dimers, tetramers, hexamers,and even higher molecular mass species, as a function of the radiation dose(PMID:17603931).It exsits as a 21-kD polypeptide specifically localized to the centrosome of interphase and mitotic cells in both HeLa and BHK cells (PMID:8248209).
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References
- Fong YW, Inouye C, Yamaguchi T, Cattoglio C, Grubisic I, Tjian R. A DNA repair complex functions as an Oct4/Sox2 coactivator in embryonic stem cells. Cell. 147(1):120-31. 2011.
- Zhang Y, Foreman O, Wigle DA. USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis. The Journal of clinical investigation. 122(12):4362-74. 2012.
- Fong YW, Ho JJ, Inouye C, Tjian R. The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells. eLife. 3:. 2014.
- Cattoglio C, Zhang ET, Grubisic I, Chiba K, Fong YW, Tjian R. Functional and mechanistic studies of XPC DNA-repair complex as transcriptional coactivator in embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America. 112(18):E2317-26. 2015.
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