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Product Name
CEMIP Polyclonal Antibody
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Description
Polyclonal antibody to CEMIP
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
CEMIP antibody; CCSP1 antibody; HYBID antibody; KIAA1199 antibody; TMEM2L antibody; cell migration inducing hyaluronan binding protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-420 of human CEMIP (NP_061159.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CEMIP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CEMIP antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - CEMIP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CEMIP antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - CEMIP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using CEMIP antibody at dilution of 1:150 (40x lens).
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Background
Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"