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Product Name
CDCA8 Polyclonal Antibody
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Description
Polyclonal antibody to CDCA8
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CDCA8 antibody; BOR antibody; BOREALIN antibody; DasraB antibody; MESRGP antibody; borealin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CDCA8 (NP_060571.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - CDCA8 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CDCA8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunohistochemistry - CDCA8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using CDCA8 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CDCA8 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using CDCA8 antibody at dilution of 1:100 (40x lens).
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Background
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"