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Product Name
CDC25B antibody
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Description
CDC25B Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, Jurkat cells, mouse brain tissue, Raji cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 60-70 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CDC25B antibody; CDC25HU2 antibody; M phase inducer phosphatase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CDC25B recombinant protein (Accession Number: NM_021873). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109097(CDC25B antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:109097(CDC25B antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-CDC25B (IP:Catalog No:109097, 4ug; Detection:Catalog No:109097 1:300) with mouse liver tissue lysate 4000ug.
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Background
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. In Jurkat cell, this antibody recognized 2 bands: 60kd(isoform 2, CDC25B2) and 70kd(isoform 4 of CDC25B).
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References
- De S, Kline D. Evidence for the requirement of 14-3-3eta (YWHAH) in meiotic spindle assembly during mouse oocyte maturation. BMC developmental biology. 13:10. 2013.
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